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2022 · Bulut — Hydrogen-rich water can reduce the formation of biogenic amines in butter

Original title: Hydrogen-rich water can reduce the formation of biogenic amines in butter.

Super-Abstract

Washing raw butter with hydrogen-rich water — prepared either by bubbling hydrogen gas or by adding magnesium — significantly reduced the formation of biogenic amines (including histamine, tryptamine, and spermidine) during 90 days of storage. This is a food-chemistry in-vitro study; it tests H₂ as a food-safety tool, not as a health intervention in humans. (Food Chemistry, 2022.)

Classified as a Mechanism / Preclinical study using Drinking (HRW). See Methodology for how we grade evidence.

Commentary

Biogenic amines such as histamine, tyramine, tryptamine, and cadaverine accumulate in dairy products through microbial decarboxylation of amino acids during storage. High levels are a food-safety concern and can cause adverse reactions in sensitive individuals. This study explores whether hydrogen-rich water (HRW), already recognized for its antimicrobial and antioxidant properties, can suppress this amine formation when used as a butter-washing agent. Two HRW preparations were compared: hydrogen-saturated water (made by bubbling H₂ gas) and magnesium-enriched water (where magnesium reacts with water to release H₂). Both consistently outperformed normal water in limiting biogenic amine accumulation over 90 days. Critically, HRW washing had no inhibitory effect on yogurt bacteria — meaning it does not simply function as a broad bactericide that would impair desirable fermentation cultures. The study opens an applied agricultural/food-technology dimension for hydrogen that is distinct from hydrogen-medicine research.

Key quotes

  1. „the lowest formation levels of biogenic amines were shown for butter samples washed with HRW (H2 water and Mg water), while the highest levels were identified for the butter samples washed with normal water.“ — the central result: HRW outperforms normal water in limiting amine accumulation
  2. „Washing butter with HRW revealed no inhibiting effect on yogurt bacteria during storage.“ — important selectivity: HRW does not harm desirable dairy cultures
  3. „The proposed method involves molecular hydrogen which is non-toxic for humans and the environment.“ — safety argument from the authors

Our assessment

This is a food-chemistry in-vitro study, not a clinical or animal experiment. Its relevance is to food safety and food technology, not to hydrogen-medicine endpoints. No human health outcomes are tested. The findings are technically interesting and potentially applicable in dairy production, but they do not speak to any therapeutic use of molecular hydrogen in the body.

Study design

Abstract

The formation of biogenic amines in food products forms a serious challenge for food producers and a hazard for consumers. Butter was washed by hydrogen-rich water (HRW) prepared using two methods, i.e. hydrogen-bubbled water (H2 water) and magnesium-incorporated water (Mg water). After 90 days of storage, the lowest formation levels of biogenic amines were shown for butter samples washed with HRW (H2 water and Mg water), while the highest levels were identified for the butter samples washed with normal water. Washing raw butter with HRW led to a significant decrease in tryptamine, 2-phenylethylamine, spermidine, and spermine formation. The lowest histamine level was shown for butter samples washed with H2 and Mg, while the highest level was found for butter washed with normal water. Washing butter with HRW revealed no inhibiting effect on yogurt bacteria during storage. The proposed method involves molecular hydrogen which is non-toxic for humans and the environment.

Source & links

Screenshot of the PubMed page

Screenshot — PubMed 35258003

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